Journal of Isfahan Dental School

Comparing the Number of Colonies of Candida Albicans in Gingival Crevicular Fluid in Diabetic and Non-Diabetic Patients with Chronic Periodontitis

Document Type : مقاله‌های پژوهشی

Authors

1 Assistant Professor, Department of Oral and Maxillofacial Medicine, Dental School, Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran.

2 Assistant Professor, Department of Periodontology, Dental School, Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran.

3 Postgraduate Student of Periodontitis, School of Dentistry, Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran.

4 Dentist, Isfahan, Iran.

Abstract

Introduction: Periodontitis is an inflammatory disease with microbial etiology; furthermore Diabetes is a metabolic disease characterized by hyperglycemia, considering Candida albicans is an opportunistic fungus that can lead to candidiasis infection; The aim of this study is to comparing the average number of colonies of candida albicans in gingival sulcus fluid in diabetic and non-diabetic patient with chronic periodontitis.
Materials & Methods: This case control study included 60 patient aged 40-60 years that leave in 4 group composed 15 individuals each group: healthy subjects, diabetic without periodontitis, non-diabetic with periodontitis and diabetic with periodontitis patients. Samples were acquired using sterile paper points from gingival sulcus and centrifuged, then cultured on chrome agar medium and incubated. Number of colonies were counted and analyzed with ANOVA and LSD tests.
Results: Counting number of colonies in different groups showed, in healthy subjects, 33.3 percent with mean 4.2 colonies and in diabetic patients without periodontitis, 66 percent with mean 13 colonies of candida albicans were grew; whereas other two groups presented candida albicans in all samples and the highest mean of colonies was found in diabetic with chronic periodontitis and non-diabetic with chronic periodontitis, 80.5 and 37.8 respectively. The average number of colonies of candida albicans was Statistical difference between groups (p value <0.001).
Conclusion: The result of this present study showed that accompaniment periodontitis and diabetes can doubled influence in increase average number of colonies of candida albicans in gingival crevicular fluid.
Key words: Candida albicans, Diabetes mellitus, Chronic periodontitis, Gingival crevicular fluid.

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Journal of Isfahan Dental School
Volume 14, Issue 2 - Serial Number 2
March 2018
Pages 145-152