Journal of Isfahan Dental School

Determination of Periopathogen Counts in Active and Passive Smokers and Nonsmoking Subjects with and without Periodontitis

Document Type : مقاله‌های پژوهشی

Authors

1 Assistant Professor, Department of Periodontology, Dental School, Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran.

2 Dentist, Isfahan, Iran.

3 Assistant Professor, Department of Operative Dentistry, School of Dentistry, Islamic Azad University Isfahan (Khorasgan) Branch, Isfahan, Iran.

4 Periodontics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

5 Postgarduate Student, Department of Periodontics, School of Dentistry, Islamic Azad University Isfahan (Khorasgan) Branch, Isfahan, Iran.

Abstract

Introduction: Periopathogens can be found in healthy subjects, too, but in areas with periodontal disease they are found in greater numbers. In the oral cavity, there are multiple mechanisms for the effect of smoking on the host‒bacteria interaction. The aim of this study was to determine the effect of smoking and periodontitis on subgingival periopathogens.
Materials & Methods: In this study, 33 patients aged 25‒65 years with moderate to severe chronic periodontitis and 33 periodontally healthy subjects aged 25‒65 years were selected. There were 11 smokers, 11 passive smokers and 11 non-smokers in each group. After periodontal examinations, GCF samples were collected from the deepest pocket of each quadrant and sent to the laboratory. The bacterial colony counts were determined and recorded. Data were analyzed with ANOVA and LSD tests (α = 0.05).
Results: Periodontitis (p value < 0.001) and smoking (p value < 0.001) had significant effects on P. gingivalis colony counts. Also, P. intermedia colony counts were significantly affected by both periodontitis (p value < 0.001) and smoking (p value < 0.001). Furthermore, F. nucleatum colony counts were significantly affected by periodontitis (p value < 0.001) and smoking status (p value < 0.001).
Conclusion: Smoking (active and passive) had significant effects on the colony counts of P.i., F.n. and P.g. colonies in periodontitis.
Key words: Fusobacterium nucleatum, Periodontitis, Porphyromonas gingivalis, Prevotella intermedia, Smoking.

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Journal of Isfahan Dental School
Volume 14, Issue 2 - Serial Number 2
March 2018
Pages 153-160