Journal of Isfahan Dental School

In Vitro Comparison of Microleakage of a Conventional, a Self-Etching and a Hydrophilic Fissure Sealant

Document Type : Original Articles

Authors

1 Postgraduate Student, Department of Restorative, School of Dentistry, Shahid Sadooghi University of Medical Sciences, Yazd, Iran.

2 Assistant Professor, Department of Pediatric, School of Dentistry, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.

3 Postgraduate Student, Department of Prosthodontic. School of Dentistry, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.

Abstract

Introduction: The preventive role of pit and fissure sealants against dental caries has clearly been shown. Two new categories of self-etching (Itena) and hydrophilic (Smartseal & Loc F) fissure sealants have recently been marketed. The aim of this study was to investigate and compare microleakage of these innovative fissure sealants with a conventional sealant material (3M).
Materials & Methods: Seventy-five sound extracted third molars from both jaws with no caries or cracks were selected for the purpose of this in vitro study and assigned to three groups. The teeth were cleaned with pumice powder and water, rinsed, dried and prepared. The occlusal pits and fissures were etched using a 37% phosphoric acid ethant gel (3M) for 20 s, rinsed for 15 s and dried. In each group, the assigned sealant material was applied over the pits and fissures and cured for 20 s: self-etching (Itena), hydrophilic (Smartseal & Loc F) and conventional sealant material (3M). The samples were then stored in 0.5% volatile fuchsine solution for 48 hours. The samples were then rinsed and sectioned in the buccolingual direction. The samples were then investigated at ×24 magnification under a stereomicroscope for depth of dye penetration. Data were analyzed with using Kruskal-Wallis and Mann-Whitney tests at a significance level of p value < 0.05.
Results: Comparison of the means showed the highest degree of microleakage in self-etching sealant (mean = 3), which was significantly different from the hydrophilic (mean = 2) and conventional (G = 0.84) fissure sealants (p value < 0.001). However, there was no statistically significant difference between conventional and hydrophilic fissure sealants (p value = 0.21).
Conclusion: The results of the present study showed that the microleakage of self-etching sealant was higher than the two other fissure sealants.
Key words: Dental leakage, Fissure sealant, Self-etching.

1. Feigal RJ, Donly KJ. The use of pit and fissure sealants. Pediatr Dent 2006; 28(2): 143-50.
2. Szoke J. [Fissure sealing. A review]. Fogorv Sz 2008; 101(4): 137-46. [In Hungarian].
3. Kuşgöz A, Tüzüner T, Ulker M, Kemer B, Saray O. Conversion degree, microhardness, microleakage and fluoride release of different fissure sealants. J Mech Behav Biomed Mater 2010; 3(8): 594-9.
4. Kiremitçi A, Yalçin F, Gökalp S. Bonding to enamel and dentin using self-etching adhesive systems. Quintessence Int 2004; 35(5): 367-70.
5. Jabbarifar SE, Ghasemi D, Barekatain M, Alizadeh F, Tahmourespoor S, Daneshnezhad M. In vitro comparison of microleakage of a self-etching fissure sealant with a flowable composite resin and a conventional fissure sealant. J Isfahan Dent Sch 2014; 10(4): 259-65. [In Persian].
6. Montanari M, Pitzolu G, Felline C, Piana G. Marginal seal evaluation of different resin sealants used in pits and fissures. An in vitro study. Eur J Paediatr Dent 2008; 9(3): 125-31.
7. Conner FA, Vandewalle KS, Bartoloni JA. Degree of microleakage of a self-etch sealant. Gen Dent 2011; 59(3): 224-8.
8. Schuldt C, Birlbauer S, Pitchika V, Crispin A, Hickel R, Ilie N. Shear bond strength and microleakage of a new self-etching/self-adhesive pit and fissure sealant. J Adhes Dent 2015; 17(6): 491-7.
9. Mehran M, Tavasoli Hojjati S, Salehi Zeinabadi M, Sheikhbahai N. A comparative evaluation of micro leakage of a resin sealant after conventional acid-etching technique, tow component self-etch and one component total-etch adhesive an in vitro study. Int J Dent 2014; 6(1): 1-3.
10. Biria M, Ghasemi A, Doroudgar K, Najafi S. An experimental micro leakage study of tow self-etch and one total-etch fissure sealants. Majallah-I-Dandanpizishki 2011; 23(3): 182-8. [In Persian].
11. Ghasemi D, Danesh Nejad M. An in vitro comparison of micro leakage of self-etching fissure sealant with flowable composite and conventional sealant. [Thesis]. Isfahan, Iran: School of Dentistry, Islamic Azad University of Isfahan (Khorasgan) Branch; 2009. [In Persian].
12. Schlueter N, Klimek J, Ganss C. Efficacy of a moisture-tolerant material for fissure sealing: a prospective randomised clinical trial. Clin Oral Investig 2013; 17(3): 711-6.
13. Bagherian A, Ahmadkhani M, Sheikhfathollahi M, Bahramabadinejad R. Microbial microleakage assessment of a new hydrophilic fissure sealant: a laboratory study. Pediatr Dent 2013; 35(7): 194-8.
14. Güçlü ZA, Dönmez N, Hurt AP, Coleman NJ. Characterisation and microleakage of a new hydrophilic fissure sealant - UltraSeal XT® hydro™. J Appl Oral Sci 2016; 24(4): 344-51.
15. Woody TL, Davis RD. The effect of eugenol-free temporay cements on micro leakage in resin bonded restorations. Oper Dent 1992; 17(5): 175-80.
16. Rosales-Leal JI, Osorio R, Toledano M, Cabrerizo-Vilchez MA, Millstein PL. Influence of eugenol contamination on the wetting of ground and etched dentin. Oper Dent 2003; 28(6): 695-9.
17. Shinohara MS, de Oliveira MT, Di Hipolito V, Giannini M, de Goes MF. SEM analysis of the acid-etched enamel patterns promoted by acidic monomers and phosphoric acids. J Appl Oral Sci 2006; 14(6): 427-35.
Journal of Isfahan Dental School
Volume 14, Issue 3 - Serial Number 3
July 2018
Pages 277-285