Cytological Changes in the Buccal Mucosa and the Lateral Border of the Tongue in Passive Smokers and Nonsmokers Using Papanicolaou Staining in Patients without Clinical Oral Lesions

Document Type : مقاله‌های پژوهشی

Authors

1 Assisant Professor, Department of Oral and Maxillofacial, School of Dentistry, Islamic Azad University Isfahan (Khorasgan) Branch, Isfahan, Iran.

2 Assisant Professor, Department of Oral and Maxillofacial Pathology, School of Dentistry, Islamic Azad University Isfahan (Khorasgan) Branch, Isfahan, Iran.

3 General Pathologist, Isfahan, Iran.

4 Dentist, Isfahan, Iran.

5 Postgraduate Student, Department of Oral and Maxillofacial Radiology, School of Dentistry, Islamic Azad University Isfahan (Khorasgan) Branch, Isfahan, Iran.

10.48305/v0i0.1328

Abstract

Introduction: Recently efforts have increased to diagnose premalignant lesions. One of these techniques is brush cytology. The aim of this study was to compare changes in the cytology samples taken from passive smokers and nonsmoking healthy patients using staining Papanicolaou technique.
Materials & Methods: In this descriptive/analytical cross-sectional study, in two groups with 23 subjects (case and control), brush cytology samples were taken from the buccal mucosa and lateral border of the tongue from passive smokers with records of the duration of exposure to cigarette smoke (hours/day), using a sterilized brush. Then samples which were already fixed by a specific spray were stained with Papanicolaou technique in the laboratory. Then the samples were evaluated for the frequency of granular cells, binuclear cells, clear nucleolus, rough chromatin, nuclear vacuoles, apoptotic cells, and nuclear and cytoplasmic polymorphism. Data were analyzed with chi-squared test and t-test (α = 0.05).
Results:There were no meaningful differences in the buccal mucosa and lateral border of the tongue between the passive smokers (p value = 0.31) and healthy control nonsmokers (p value = 0.26) in the frequency of micronucleus, clear nucleus, polymorphism, inflammation, and keratin overproduction. The mean years of cigarette consumption in the two groups of passive smokers with and without binuclear cells were significantly different (p value = 0.004).
 Conclusion: The risk of cytologic changes might increase in passive smokers with an increase in exposure to cigarette smoke. However, cigarette was not a potential risk for mucosal changes in passive smokers.
Key words: Buccal mucosa, Cytology, Smoking.

References

1. Kumar V, Abbas AK, Aster JC. Robbins and Cotran pathologic basis of disease. 9th ed. Philadelphia, PA: Elsevier/Saunders; 2015.
2. Mehrotra R. The role of cytology in oral lesions: a review of recent improvement. Diagn Cytopathol 2012; 40(1): 73-83.
3. Messadi DV, Wilder-Smith P, Wolinsky L. Improving oral cancer survival: the role of dental providers. J Calif Dent Assoc 2009; 37(11): 789-98.
4. Usta U, Berberoglu U, Helvaci E, Altaner S, Sut N, Ozdemir G. Evaluation of cytological alterations in normal-appearing oral mucosal epithelia of smokers and non smokers via AgNOR count and nuclear. Trakya Univ Tip Fak Derg 2008; 25(2): 110-60.
5. Regezi JA, Sciubba JJ, Jordan RCK. Oral pathology: clinical pathologic correlations. 7th ed. St. Louis: Elsevier; 2017.
6. Sugerman PB, Savage NW. Exfoliative cytology in clinical oral pathology. Aust Dent J 1998; 41(2): 71-4.
7. Cowson RA, Odell EW. Cawson's essentials of oral pathology and oral medicine. 8th ed. New York, UK: Churchill Livingstone/Elsevier; 2008. p. 7-9, 252-3.
8. Neville BW, Damm DD, Allen CM, Chi AC. Oral and maxillofacial pathology. 4th ed. St. Louis: Elsevier; 2016. p. 409-11, 419-21.
9. Maziak W, Eissenberg T, ward KD. Pattern of waterpipe use and dependence: Implications for intervention development. Pharmacol Biochem Behav 2005; 80(1): 173-9.
10. Etzel RA. A review of the use of saliva cotinine as a marker of tobacco smoke exposure. Prev Med 1990; 19(2): 190-7.
11. Jones LL, Hashim A, Mckeever T, Cook DG, Britton J, Leonardi-Bee J. Parental and household smoking and the increased risk of bronchitis, bronchiolitis and other lower respiratory infections in infancy: systematic review and meta-analysis. Respir Res 2011; 12(1): 5.
12. Bartal M. Health effects of tobacco use and exposure. Monaldi Arch Chest Dis 2001; 56(6): 545-54.
13. Erdemir EO, Sonmez IS, Oba AA, Bergestrom J, Caglayan O. Periodontal health in children exposed to passive smoking. J Clin Periodontal 2010; 37(2): 160-4.
14. Hanioka T, Tanaka K, Ojima M, Yuuki K. Association of melanin pigmentation in the gingive of children with parents who smoke. Pediatrics 2005; 116(2): e186-90.
15. Glick M. Burket's oral medicine. 12th ed. Connecticut: People's Medical Publishing House USA; 2015.
16. Edris AMM, Ahmed HG, Mohammad EA. Accuracy of oral exfoliative cytology in Sudanese patients undergoing oral biopsy. RSBO 2011; 8(3); 225-60.
17. Azimi-Hoseaini S, Mashhadi-Abbas F, Kharrazifard M J, Ebrahimifard T, Rafieian N. Comparison of cytological findings of the buccal mucosa between smokers and non smokers. The Journal of Islamic Dental Association of IRAN (JIDA) 2011; 23(1): 10-16. [In Persian].
18. Cancado RP, Yurgel LS, Filho MS. Evaluation of the nuclear organizer region associated protein in esfoliative cytology of normal buccal mucosa. Effect of smoking. Oral Oncol 2001; 37(5): 446-54.
19. Schwartz JL, Muscat JE, Baker V, Larios E, Stephenson GD, Guo W, et al. Oral cytology assessment by flow cytometry of DNA adducts, aneuploidy, proliferation and apoptosis shows differences between smokers and non-smokers. Oral Oncol 2003; 39(8): 842-54.
20. Ahmed HG, Ebnoof SO, Hussein MO, Gbreel AY. Oral epithelial atypical changes in apparently healthy oral mucosa exposed to smoking, alcohol, peppers and hot meals, using the AgNOR and Papanicolaou staining techniques. Diagn Cytopathol 2010; 38(7): 489-95.
21. Ahmed HG, Babiker AA. Assessment of cytological atypia, AgNOR and nuclear of normal oral mucosa exposed to toombak and smoking. Rare Tumors 2009; 1(1): e18.
22. Hijazi M, Jentsch H, Al-Sanabani J, Tawfik M, Remmerbach TW. Clinical and cytological study of the oral mucosa of smoking and non-smoking qat chewers in Yemen. Clin Oral Investig 2016; 20(4): 771-9.
23. Hashemipour MA, Aghababaie M, Mirshekari TR, Asadi-Shekaari M, Tahmasbi-Arashlow M, Tahmasbi-Arashlow F, et al. Exfoliative cytology of oral mucosa among smokers, opium addicts and non-smokers: a cytomorphometric study. Arch Iran Med 2013; 16(12): 725-30.
24. Shinohara MS, de Oliveira MT, Di Hipolito V, Giannini M, de Goes MF. SEM analysis of the acid-etched enamel patterns promoted by acidic monomers and phosphoric acids. J Appl Oral Sci 2006; 14(6): 427-35.

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