In vitro Comparison of Three Different Methods of Denture Base Acrylic Resin Process in Attachment and Colonization of Candida Albicans
Document Type : مقالههای پژوهشی
Authors
1 Dentist, Isfahan, Iran
2 Department of Oral and Maxillofacial Diseases, School of Dentistry, Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran
3 Department of Prosthodontics, School of Dentistry, Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran
4 Post Graduate, Department of Prosthodontics, School of Dentistry, Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran
10.48305/v0i0.1436
Abstract
Introduction: Candidiasis is the most common fungal infection in oral cavity, usually caused by Candida albicans. Since one of the most common complications of denture wearing is denture stomatitis, presence of opportunistic microorganisms such as C. albicans and colonization of denture base by these microorganisms, the aim of this study was to compare the effects of three different methods of acrylic resin processing on adherence and colonization of C. albicans.
Materials and Methods: In this experimental study, 60 square acrylic resin samples, measuring 10×10×10 mm3 (Ivoclar Vivadent, Liechtenstein) were processed with three methods of preparation: cold-cured [CC], heat-cured [HC] and injection-molded [IM]. Yeast suspensions were incubated on the surface of resin blocks for 40 minutes and then washed with 1 mL of sterile saline solution; 20 µL of this solution were inoculated on Sabouraud dextrose agar and incubated at 37 C. The colony counts were determined after 24 and 48 hours. At 60- and 120-minute intervals the colony counts were determined again. Data were analyzed with SPSS 20, using two-way ANOVA and paired t-test (α = 0.05).
Results: The method of preparation of acrylic resin (p value = 0.001) and duration of proximity of acrylic resin with the yeasts (p value < 0.001) were significant. The lowest colony counts were observed in the cold-cured method. In all these three methods and durations of proximity of acrylic resin and C. albicans, the colony counts at 48 hours were significantly higher than those at 24 hours (p value < 0.001).
Conclusion: The acrylic resin preparation method affected the adhesion and colonization of C. albicans. Considering the lower colonization in cold-cured acrylic resin, it is the most appropriate acrylic resin, especially for those with poor oral hygiene or those susceptible to fungal infections.
Key words: Candida albicans, Acrylic resin, Denture stomatitis, Colonization
Materials and Methods: In this experimental study, 60 square acrylic resin samples, measuring 10×10×10 mm3 (Ivoclar Vivadent, Liechtenstein) were processed with three methods of preparation: cold-cured [CC], heat-cured [HC] and injection-molded [IM]. Yeast suspensions were incubated on the surface of resin blocks for 40 minutes and then washed with 1 mL of sterile saline solution; 20 µL of this solution were inoculated on Sabouraud dextrose agar and incubated at 37 C. The colony counts were determined after 24 and 48 hours. At 60- and 120-minute intervals the colony counts were determined again. Data were analyzed with SPSS 20, using two-way ANOVA and paired t-test (α = 0.05).
Results: The method of preparation of acrylic resin (p value = 0.001) and duration of proximity of acrylic resin with the yeasts (p value < 0.001) were significant. The lowest colony counts were observed in the cold-cured method. In all these three methods and durations of proximity of acrylic resin and C. albicans, the colony counts at 48 hours were significantly higher than those at 24 hours (p value < 0.001).
Conclusion: The acrylic resin preparation method affected the adhesion and colonization of C. albicans. Considering the lower colonization in cold-cured acrylic resin, it is the most appropriate acrylic resin, especially for those with poor oral hygiene or those susceptible to fungal infections.
Key words: Candida albicans, Acrylic resin, Denture stomatitis, Colonization
1. Aslanimehr M, Rezvani S, Mahmoudi A, Moosavi N. Comparison of Candida albicans adherence to conventional acrylic denture base materials and injection molding acrylic materials. J Dent (Shiraz). 2017; 18(1): 61-4.
2. Filler SG. Candida-host cell receptor-ligand interactions. Curr Opin Microbiol 2006; 9(4): 333-9.
3. Kang SH, Lee HJ, Hong SH, Kim KH, Kwon TY. Influence of surface characteristics on the adhesion of Candida albicans to various denture lining materials. Acta Odontol Scand 2013; 71(1): 241-8.
4. Rajaram A, Manoj SS. Influence of 3 different forms of a commercially available denture adhesive material on the growth of Candida species: An in vitro study. J Prosthet Dent 2017; 118(3): 379-85
5. Chen H, Han Q, Zhou X, Zhang K, Wang S, Xu HHK, et al. Heat-polymerized resin containing dimethy laminododecyl methacrylate inhibits Candida albicans biofilm. Materials (Basel) 2017; 10(4): 431.
6. Zarb GA, Hobkirk J, Eckert S, Jacob R. Prosthodontic treatment for edentulous patients: complete dentures and implant-supported prostheses. 13th ed. St. Louis: Elsevier Health Sciences; 2013. p. 44-5, 133-7.
7. Hahnel S, Rosentritt M, Handel G, Burgers R. In vitro evaluation of artificial ageing on surface properties and early Candida albicans adhesion to prosthetic resins. J Mater Sci Mater Med 2009; 20(1): 249-55.
8. Kulak-Ozkan Y, Kazazoglu E, Arikan A. Oral hygiene habits, denture cleanliness, presence of yeasts and stomatitis in elderly people. J Oral Rehabil 2002; 29(3): 300-4.
9. Little JW, Falace D, Miller C, Rhodus NL. Dental management of the medically compromised patient. 9th ed. St. Louis: Elsevier; 2018.
10. Koch C, Burgers R, Hahnel S. Candida albicans adherence and proliferation on the surface of denture base materials. Gerodontology 2013; 30(4): 309-13.
11. Young B, Joes A, Cameron D, McCord F, Murray C, Bagg J, et al. Attachment of Candida albicans to denture base acrylic resin processed by three different methods. Int J Prosthodont 2009; 22(5): 488-9.
12. Sadeghpour M, Falahati M, Ashrafi M, Shirian AA. The comparison of the bayer acryl and acropars acryl effect on the adhesion of Candida albicans. Razi J Med Sci 2011; 18(89):20-6. [In Persian].
13. Uchimaru M, Sakai T, Moroi R, Shiota S, Shibata Y, Deguchi M, et al. Antimicrobial and antifungal effects of tissue conditioners containing a photocatalyst. Dent Mater J 2011; 30(5): 691-9.
14. Radford DR, Sweet SP, Challacombe SJ, Walter JD. Adherence of Candida albicans to denture-base materials with different surface finishes. J Dent 1998; 26(7): 577-83.
15. Nevzatoğlu EU, Ozcan M, Kulak-Ozkan Y, Kadir T. Adherence of Candida albicans to denture base acrylics and silicone-based resilient liner materials with different surface finishes. Clin Oral Investig 2007; 11(3): 231-6.
16. Nomura T, Suzuki T, Furuya J, Shimoyama Y, Sasaki M, Kimura Sh. In vitro adherence of Candida albicans to acrylic resin with different surface status. In: Sasaki K, Suzuki O, Takahashi N. editors. Interface oral health science. Berlin, Germany: Springer; 2012. p. 145-6.
17. Park SE, Blissett R, Susarla SM, Weber HP. Candida albicans adherence to surface-modified denture resin surface. J Prosthodont 2008; 17(5): 365-9.
18. Bregula L, Trzeciak H, Nolewajka-Lasak I. The study of adhesion of Candida albicans to the selected acrylic resins. Med Dosw Mikrobiol 2006; 58(1): 67-71.
19. Celebi N, Yuzugullu B, Canany S, Yucel U. Effect of polymerization methods on the residual monomer level of acrylic resin denture base polymers. Polymers for advanced technologies 2008; 19(3): 201-6.
20. Anderson GC, Schulte JK, Arnold TG. Dimensional stability of injection and conventional processing of denture base acrylic resin. J Prosthet Dent 1988; 60(3): 394-8.
21. Strohaver RA. Comparison of changes in vertical dimension between compression and injection molded complete dentures. J Prosthet Dent 1989; 62(6): 716-8.
22. Hugget R, Zissis A, Harrison A, Dennis A. Dimensional accuracy and stability of acrylic resin denture bases. J Prosthet Dent 1992; 68(4): 634-40
23. Nogueria SS, Ogle RE, Davis EL. Comparison of accuracy between compression-and injection molded complete dentures. J Prosthet Dent 1999; 82(3): 291-300.
24. Dhanraj M, Robin P, Nallasamy D. Ariga P, Anand S. Influence of polymerization shrinkage on denture base distortion - a systematic review. Indian Journal of Multidisciplinary Dentistry 2013; 3(2): 687-94.
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