مجله دانشکده دندان‌پزشکی اصفهان

بررسی مقایسه‌ای خاصیت ضد باکتریای دو ماده‌ی جدید پرکننده‌ی انتهای ریشه‌ی دندان و MTA بر روی باکتری انتروکوکوس فکالیس

نوع مقاله : Original Articles

چکیده

مقدمه: جهت جراحی اندودنتیک، نیازمند موادی با فعالیت ضد باکتریایی هستیم. هدف از این مطالعه، بررسی مقایسه‌ای فعالیت ضد باکتریایی دو سمان جدید پر کننده‌ی انتهای ریشه‌ی دندان و MTA (Mineral trioxide aggregate) بر انتروکوکوس فکالیس بود.
مواد و روش‌ها: این مطالعه‌ی تجربی- آزمایشگاهی، به روش تست تماس مستقیم DCT (Direct contact test) صورت گرفت. ترکیبات مورد مطالعه سمانی با بیس کلسیم آلومینات (Calcium aluminate α-aluminate cement) CAAC و سمانی با بیس کلسیم سیلیکات که مخلوطی از (Wollastonite and CAAC) WOLCA با MTA بود. هر یک از سمان‌ها بلافاصله پس از مخلوط شدن (ست نشده)، 3 روز و 1 هفته پس از زمان ست شدن‌شان، تهیه شده و در کف 4 حفره از پلیت 96 حفره‌ای قرار داده شدند (گروه a). 10 میکرو لیتر از سوسپانسیون باکتری انتروکوکوس فکالیس به غلظت 0/5 مک فارلند و 245 میکرو لیتر BHI به آنها اضافه شد و در حفرات گروه a و حفرات گروه b (حفرات خالی) اضافه گردید. در نهایت میزان رشد باکتری در هر یک از حفرات گروه b هر نیم ساعت به مدت 7 ساعت و یک‌بار 24 ساعت بعد، توسط دستگاه اسپکتروفوتومتر مشاهده و ثبت شد. داده‌های به دست آمده با روش‌های آماری آنالیز واریانس برای داده‌های مکرر و آنالیز واریانس یک‌طرفه و آزمون توکی تحلیل آماری شدند (0/05 = α).
 یافته‌ها: نتایج آزمون توکی، نشان داد که بین MTA تازه مخلوط شده و MTA یک هفته پس از ست شدن، تفاوت معنی‌دار وجود دارد (0/033 = p value). همچنین بین MTA یک هفته پس از ست شدن و CAAC یک هفته پس از ست شدن و بین MTA یک هفته پس از ست شدن و WOLCA سه روز پس از ست شدن نیز تفاوت معنی‌دار وجود داشت (به ترتیب 0/039 = p value و 0/043 = p value). اما سایر گروه‌ها تفاوت معنی‌داری را نشان ندادند.
نتیجه‌گیری: به دلیل شباهت فعالیت ضد باکتریایی دو سمان مورد آزمایش، با MTA و حتی بیشتر بودن فعالیت ضد باکتریایی CAAC یک هفته پس از ست شدن و WOLCA سه روز پس از ست شدن، نسبت به MTA تهیه شده یک هفته پس از ست شدن، می‌توان این سمان‌ها را جهت بررسی‌های آزمایشگاهی و کلینیکی بیشتر در آینده مورد توجه قرار داد.
کلید واژه‌ها: انتروکوکوس فکالیس، فعالیت ضد باکتریایی، مینرال تری اکسید اگریگیت، تست تماس مستقیم.

عنوان مقاله [English]

Comparative evaluation of antibacterial activities of two new root-end filling materials and MTA against Enterococcus faecalis

چکیده [English]

Introduction: Antibacterial agents are required for endodontic surgery. The aim of this study was to compare the antibacterial activities of two new root-end filling materials and MTA against Enterococcus faecalis.
Materials & Methods: This in vitro study was carried out using direct contact test (DCT). The materials studied consisted of a calcium aluminate-based cement (CAAC, calcium aluminate α-aluminate cement) and a calcium silicate-based cement which was a mixture of wollastonite, CAAC (WOLCA) and MTA. Each cement was prepared immediately after mixing (not set), 3 days and 1 week after their setting and placed at the bottom of 4 wells from a 96-well plate (group A). Approximately 10 μL of Enterococcus faecalis suspension at 0.5 McFarland concentration and 245 μL of BHI were added to the wells in group A and empty wells in group B. Finally, the growth rate of bacteria in the wells in each group was monitored every 30 minutes for 7 hours and once after 24 hours using the spectrophotometry technique. Data were analyzed with ANOVA for repeated data and with one-way ANOVA and post hoc Tukey tests (p value = 0.05).
Results: Tukey tests showed a significant difference between the freshly mixed MTA and the MTA one week after its setting (p value = 0.033). In addition, there were significant differences between MTA one week after its setting and CAAC one week after its setting and between MTA one week after its setting and WOLCA three days after its setting (p value = 0.039 and p value = 0.043, respectively); however, there were no significant differences between the other groups.
Conclusion: Due to the similarity of the antibacterial activities of the tested cements and MTA and even higher antibacterial activities of CAAC one week after its setting, and WOLCA three days after its setting compared to MTA one week after its setting, these cements can be considered for further in vitro and clinical studies in future.
Key words: Antibacterial activity, Direct contact test, Enterococcus faecalis, Mineral trioxide aggregate.

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مجله دانشکده دندان‌پزشکی اصفهان
دوره 15، شماره 3 - شماره پیاپی 3
مرداد 1398
صفحه 330-339