Journal of Isfahan Dental School

The in vitro Survey of the Effect of Protease Enzyme on Reduction of Biofilm Producted by Streptococcus Mutans Isolated from Dental Plaque

Document Type : مقاله‌های پژوهشی

Authors

1 MSc Student of Microbiology, Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

2 Assistant professor, Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

Abstract

Introduction: Bacteria growing in dental plaque have increasing resistant to antimicrobial agents. The present study aimed for reduction of biofilm formation by streptococcus mutans isolated from dental plaque by protease enzyme.
Materials and Methods: Streptococcus mutans from dental plaque was isolated in Blood agar medium and then was identified by biochemical tests and sequencing of 16S rRNA coding gene. Bacterial biofilms were formed in wells of microtiter plate in Triptic Soy Broth medium supplemented with 1% sucrose after 48 hrs incubation in 37ºC. Then the volumes equal to 1.12, 2.24 and 4.4 units per well of protease was separately added to recently formed biofilms in different wells based on the manufacturer protocol and effects of them was studied on reduction of biofilms in 60, 90, 120, 150 and 210 minutes by spectrophotometer. Mean differences between independent groups were compared with Kruskal-Wallis test and SPSS25 software statistical tests and paired means were compared with Mann-Whitney test at 95% significance level.
Results: Streptococcus mutans was isolated from dental plaque had 98.90% similarity to LPB0225 strain and had intermediated adherence. Reduction of biofilm percentage by Streptococcus mutans LPB0225 was observed in every of units of enzyme and in every periods of times. The most biofilm percentage reduction by Streptococcus mutans LPB0225 was observed in 1.12 units of enzyme in 90 minutes with 97% reduction. Biofilm percentage reduction in 1.12 units of enzyme in 60, 150 and 210 minutes had significantly differences with each other (p value = 0.0008).
Conclusion: The protease enzyme was able to reduce the dental plaque of Streptococcus mutans LPB0225. The survey on the effect of this enzyme on mixed dental plaques is suggested to control dental caries and plaques created on dentures, and to prevent periodontal diseases caused by dental plaque.
Key words: Streptococcus mutans, Protease enzyme, Biofilm, Dental plaque

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Journal of Isfahan Dental School
Volume 16, Issue 4 - Serial Number 4
January 2021
Pages 437-447