Journal of Isfahan Dental School

Comparison between the effect of A-PRF and L-PRF on the number of fibroblasts adhered to collagen membrane

Document Type : مقاله‌های پژوهشی

Authors

1 Postgraduate Student, Department of Periodontics, School of Dentistry, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

2 Professor, Department of Periodontics, School of Dentistry, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

3 Assistant Professor, Department of Periodontics, School of Dentistry, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

Abstract

Introduction: Growth factors and cytokines in the platelet play a major role in the progression of inflammation and healing processes, therefore A-PRF and L-PRF as platelet drive ingredients are widely used in periodontal treatments. The purpose of this study is to compare the effect of A-PRF and L-PRF on the number of gingival fibroblasts adhered to collagen membrane.
Materials and Methods: In this In-vitro study conducted in winter of 2021 at the Iranian biological resource center, the membrane was cut into five pieces (5x5 mm) and placed at the bottom of a 5 well plate. 1 milliiiter of (A-PRF) was added to two wells in each group. And a similar amount of L-PRF was added to two wells in the other two groups. The last well was left A-PRF or L-PRF free. Gingival fibroblasts were seeded in all the wells. Cell adhesion was evaluated by means of a Field Emission Scanning Electron Microscope after 24 hours of incubation. The Data were analyzed by, one way and two-way ANOVA and Scheffe test (p value < 0.001).
Results: Based on ANOVA there was a statistically significant difference In the A-PRF + group and L-PRF+ group (12.50 and 13.57) compared to the control group (4.67) (p value < 0.001).and Scheffe analysis showed no statistically significant difference between A-PRF+ or L-PRF+ groups (p value = 0.307).
Conclusion: A-PRF and L-PRF are both effective in the adhesion of fibroblasts cells to collagen membranes, although there is no difference between A-PRF and L-PRF in the adhesion of fibroblast cells to collagen membranes.
Keywords: Guided Tissue Regeneration; collagen membrane; Cell Adhesion; PRF; fibroblast

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Journal of Isfahan Dental School
Volume 19, Issue 1 - Serial Number 1
June 2023
Pages 9-19