مجله دانشکده دندان‌پزشکی اصفهان

تأثیر ضد میکروبی دو سیستم تک فایلی در برابر یک سیستم چرخشی چند فایلی در کانال‌های عفونی

نوع مقاله : مقاله‌های پژوهشی

چکیده

مقدمه: از آن‌جایی که میکروارگانیسم‌ها نقش اساسی در اتیولوژی بیماری‌های پالپ و پری‌اپیکال ایفا می‌کنند و کاهش و یا حذف جمعیت باکتریایی داخل کانال از اصلی‌ترین اصول درمان ریشه‌ی موفق می‌باشد، هدف از مطالعه‌ی حاضر، ارزیابی تأثیر ضدمیکروبی دو سیستم تک فایلی در برابر یک سیستم چرخشی چندفایلی در کانال‌های آلوده با باکتری Enterococcus faecalis بود.
مواد و روش‌ها: در این مطالعه‌ی تجربی آزمایشگاهی، کانال ریشه‌ی 48 دندان پرمولر مندیبل توسط باکتری Enterococcus faecalis به مدت ۳۰ روز آلوده شد. نمونه‌ها به صورت تصادفی به سه گروه تقسیم شدند: رسیپروک، نئولیکس و ام تو (۱۶ = n). گروه شاهد منفی شامل چهار دندان آلوده شده بود که تحت آماده‌سازی قرار نگرفت. نمونه‌های باکتریایی قبل و بعد از آماده‌سازی جمع‌آوری گردید و بررسی کمی باکتری توسطquantitative polymerase chain reaction)) qPCR صورت گرفت. آنالیزهای آماری توسط آزمون‌های Mann-Whitney و Kruskal-Wallis انجام شد. سطح معنی‌داری به صورت (0/05 > p value) به دست آمد.
یافته‌ها: در مقایسه‌ی میانگین تعداد باکتری‌ها قبل و بعد از آماده‌سازی، بین سه گروه رسیپروک، نئولیکس و ام تو، بعد از آماده‌سازی تعداد باکتری‌ها بطور معنی‌داری افزایش داشت (0/001 > p value). ولی در گروه شاهد منفی، تفاوت معنی‌داری در تعداد باکتری‌ها قبل و بعد از آماده‌سازی وجود نداشت (0/144 = p value).
نتیجه گیری: سیستم‌‌های تک فایلی آماده‌سازی کانال به اندازه‌ی سیستم چرخشی چند فایلی در کاهش باکتری‌های E. Faecalis کانال مؤثر بودند.
کلید واژه‌ها: Enterococcus faecalis؛ ضدمیکروبی؛ اندودانتیکس. 

عنوان مقاله [English]

Antimicrobial Effect of Two Single File Systems versus One Multiple Rotary Instrumentation on Infected Root Canals

چکیده [English]

Introduction: Since microorganisms play a fundamental role in the ethology of pulp and periapical diseases, and reduction or elimination of the intracanal population, is a main principles of successful root canal treatment. The aim of this study was the in vitro comparison of bacterial population reduction in root canal by mechanical instrumentation, using two single file systems and multi-file rotary system in root canals infected with Enterococous Faecalis.
Materials and Methods: In this experimental laboratory study, the root canals of 48 human mandibular premolar teeth were selected and infected with a culture of E.faecalis for 30 days. Then the specimens were randomly divided into 3 groups (n = 16): Reciproce, Neolix and Mtwo files. Four other teeth that were contaminated and not instrumented, were used as the control group. Samples were taken before (S1) and after instrumentation (S2). Bacterial quantification was performed using quantitive PCR. Statistical analyses were performed using the Mann-Whitney and Kruskal-Wallis tests.
Results: Comparing the average number of bacteria before and after preparation, the number of bacteria increased significantly between the three groups of Reciproc, Neolix and MTO after preparation (p value < 0.001). However in the control group, there was no significant difference in the number of bacteria before and after preparation (p value = 0.144).
Conclusion: Single file canal preparation systems are as effective as multifile rotary system in reducing the bacterial load of canals.
Keywords: Enterococcus faecalis; Antimicrobial; Endodontics

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مجله دانشکده دندان‌پزشکی اصفهان
دوره 19، شماره 2 - شماره پیاپی 2
تیر 1402
صفحه 133-140