Journal of Isfahan Dental School

Antimicrobial Effect of Two Single File Systems versus One Multiple Rotary Instrumentation on Infected Root Canals

Document Type : مقاله‌های پژوهشی

Authors

1 Endodontics, Department of Endodontics, School of Dentistry, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

2 Assistant Professor, Department of Endodontics, School of Dentistry ,Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

3 Associate Professor, Department of Basic Medical Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

4 Postgraduate Student, Department of Endodontics, School of Dentistry ,Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

Abstract

Introduction: Since microorganisms play a fundamental role in the ethology of pulp and periapical diseases, and reduction or elimination of the intracanal population, is a main principles of successful root canal treatment. The aim of this study was the in vitro comparison of bacterial population reduction in root canal by mechanical instrumentation, using two single file systems and multi-file rotary system in root canals infected with Enterococous Faecalis.
Materials and Methods: In this experimental laboratory study, the root canals of 48 human mandibular premolar teeth were selected and infected with a culture of E.faecalis for 30 days. Then the specimens were randomly divided into 3 groups (n = 16): Reciproce, Neolix and Mtwo files. Four other teeth that were contaminated and not instrumented, were used as the control group. Samples were taken before (S1) and after instrumentation (S2). Bacterial quantification was performed using quantitive PCR. Statistical analyses were performed using the Mann-Whitney and Kruskal-Wallis tests.
Results: Comparing the average number of bacteria before and after preparation, the number of bacteria increased significantly between the three groups of Reciproc, Neolix and MTO after preparation (p value < 0.001). However in the control group, there was no significant difference in the number of bacteria before and after preparation (p value = 0.144).
Conclusion: Single file canal preparation systems are as effective as multifile rotary system in reducing the bacterial load of canals.
Keywords: Enterococcus faecalis; Antimicrobial; Endodontics

1. Kakehashi S, Stanley HR, Fitzgerald RJ. The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats. Oral Surg Oral Med Oral Pathol 1965; 20: 340-49.
2. Bergenholtz G. Micro-organisms from necrotic pulp of traumatized teeth. Odontol Revy 1974; 25(4): 347-58.
3. Coldero G, MacHugh S, MacKanzie D, Saunders WP. Reduction in intracanal bacteria during root canal preparation with and without apical enlargment. Int Endod J 2002; 35(5): 437-46.
4. Stuart CH, Schwartz SA, Beeson TJ, Owatz CB. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod 2006; 32(2): 93-8.
5. Ferrer-Luque CM, Bejarano I, Ruiz-Linares M, Baca P. Reduction in Enteroccocus faecalis counts - a comparison between rotary and reciprocating systems. Int Endod J 2014; 47(4): 380-6.
6. Nakamura VC, Candeiro GT, Cai S, Gavini G. Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals. Braz oral Res 2015; 29: S1806-83242015000100224.
7. Alves FR, Rôças IN, Almeida BM, Neves MAS, Zoffoli J, Siqueira Jr JF. Quantitative molecular and culture analyses of bacterial elimination in oval-shaped root canals by a single-file instrumentation technique. Int Endod J 2012; 45(9): 871-7.
8. Siqueira Jr JF, Rôças IN. Polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004; 97(1): 85-94.
9. Marinho ACS, Martinho FC, Gonçalves LM, Rabang HRC, Gomes BPFA. Does the Reciproc file remove root canal bacteria and endotoxins as effectively as multifile rotary systems? Int Endod J 2015; 48(6): 542-8.
10. Martinho FC, Gomes APM, Fernandes AMM, Ferreira NS, Endo MS, Freitas LF, et al. Clinical comparison of the effectiveness of single-file reciprocating systems and rotary systems for removal of endotoxins and cultivable bacteria from primarily infected root canals. J Endod 2014; 40(5): 625-9.
11. Vivacqua-Gomes N, Gurgel-Filho ED, Gomes BPFA, Ferraz CCR, Zaia AA, Souza-Filho FJ. Recovery of Enterococcus faecalis after single- or multiple-visit root canal treatments carried out in infected teeth ex vivo. Int Endod J 2005; 38(10): 697-704.
12. Machado MEL, Nabeshima CK, Leonardo MFP, Reis FAS, Britto MLB, Cai S. Influence of reciprocating single-file and rotary instrumentation on bacterial reduction on infected root canals. Int Endod J 2013; 46(11): 1083-7.
13. Foschi F, Nucci C, Montebugnoli L, Marchionni S, Breschi L, Malagnino VA, et al. SEM evaluation of canal wall dentine following use of Mtwo and ProTaper NiTi rotary instruments. Int Endod J 2004; 37(12): 832-9.
14. de Lima Machado ME, Sapia LAB, Cai S, Martins GHR, Nabeshima CK. Comparison of two rotary systems in root canal preparation regarding disinfection. J Endod 2010; 36(7): 1238-40.
15. Yared G, Ramli GA. Single file reciprocation: a literature review. ENDO (Lond Engl) 2013; 7(3): 171-8.
16. Bürklein S, Benten S, Schäfer E. Shaping ability of different single-file systems in severely curved root canals of extracted teeth. Int Endod J 2013; 46(6): 590-7.
17. Bürklein S, Hinschitza K, Dammaschke T, Schäfer E. Shaping ability and cleaning effectiveness of two single-file systems in severely curved root canals of extracted teeth: Reciproc and WaveOne versus Mtwo and ProTaper. Int Endod J 2012; 45(5): 449-61.
18. Bürklein S, Schäfer E. Apically extruded debris with reciprocating single-file and full-sequence rotary instrumentation systems. J Endod 2012; 38(6): 850-2.
19. Marinho ACS, Martinho FC, Zaia AA, Ferraz CCR, de Almeida Gomes BP. Influence of the apical enlargement size on the endotoxin level reduction of dental root canals. J Appl Oral Sci 2012; 20(6): 661-6.
20. Martinho FC, Chiesa WMM, Marinho ACS, Zaia AA, Ferraz CCR, Almeida JFA, et al. Clinical investigation of the efficacy of chemomechanical preparation with rotary nickel-titanium files for removal of endotoxin from primarily infected root canals. J Endod 2010; 36(11): 1766-9.
21. Chen L, Li X, Zhou X, Zeng J, Ren Z, Lei L, et al. Inhibition of enterococcus faecalis growth and biofilm formation by molecule targeting cyclic di-AMP synthetase activity. J Endod 2018; 44(9): 1381-8.
22. Ghoddusi J, Arian E, Golmohammadi M, Gharechahi M, Moushekhian S. Intratubular Enterococcus faecalis viability assessment following root canal instrumentation with rotary and reciprocating systems via fluorescence microscopy. J Dent Res Dent Clin Dent Prospects 2020; 14(4): 214-7.
23. Neves MAS, Provenzano JC, Rôças IN, Siqueira Jr JF. Clinical antibacterial effectiveness of root canal preparation with reciprocating single-instrument or continuously rotating multi-instrument systems. J Endod 2016; 42(1): 25-9.
24. Martinho FC, Gomes BPFA. Quantification of endotoxins and cultivable bacteria in root canal infection before and after chemomechanical preparation with 2.5% sodium hypochlorite. J Endod 2008; 34(3): 268-72.
25. Siqueira Jr JF, Guimarães-Pinto T, Rôças IN. Effects of chemomechanical preparation with 2.5% sodium hypochlorite and intracanal medication with calcium hydroxide on cultivable bacteria in infected root canals. J Endod 2007; 33(7): 800-5.
Journal of Isfahan Dental School
Volume 19, Issue 2 - Serial Number 2
July 2023
Pages 133-140