مجله دانشکده دندان‌پزشکی اصفهان

تأثیر درمان با لیزر کم توان بر روی سطح IL-17 مایع شیار لثه‌ای طی درمان ارتودنسی

نوع مقاله : مقاله‌های پژوهشی

چکیده

مقدمه: با توجه به نقش مدیاتورهای التهابی در حرکات ارتودنسی و ایجاد بیماری‌های پریودنتال و با توجه به نقش ‌IL-17 به عنوان واسطه‌ی التهابی در درمان‌های ارتودنسی و تأثیر درمان با لیزر کم توان به عنوان درمان جانبی جدید و مناسب، هدف از این مطالعه، تأثیر درمان با لیزر کم توان بر روی سطح IL-17 در مایع شیار لثه‌ای طی درمان ارتودنسی بود.
مواد و روش‌ها: در این مطالعه‌ی کارآزمایی بالینی که در دانشکده‌ی دندان‌پزشکی دانشگاه آزاد اسلامی واحد اصفهان (خوراسگان) در سال تحصیلی 98-1397 انجام شد، تعداد 12 بیمار نیاز به درمان ارتودنسی همراه با کشیدن دندان انتخاب شدند. یک سمت برای تابش لیزر و یک سمت به عنوان کنترل در نظر گرفته شد. تابش لیزر در تمام طول شیار لثه‌ای در روزهای 0، 2، 18 و 30 پس از درمان ارتودنسی انجام و نمونه‌گیری صورت گرفت. سطح IL-17 نمونه‌ها در آزمایشگاه و با استفاده از دستگاه ELISA Reader تعیین گردید. داده‌ها با آزمون‌های آماری t-test و ANOVA تجزیه و تحلیل شدند (0/05 = α).
یافته‌ها: میانگین IL-17 روز صفر (0/62 = p value) و روز دوم بعد از ارتودنسی (0/19 = p value) بین دو سمت کنترل و درمان با لیزر، اختلاف معنی‌داری نداشت اما در روزهای 18 و 30 بعد از ارتودنسی در گروه درمان با لیزر به طور معنی‌داری کمتر از گروه شاهد بود (0/001 p value <). میانگین IL-17 در هر دو گروه در طی دوران درمان، کاهش داشت.
نتیجه‌گیری: نلیزر کم توان دیود در کاهش میزان التهاب مؤثر بود و سطح اینترلوکین 17 در هر دو سمت دهان با گذشت زمان به تدریج کاهش ‌یافت که این کاهش در سمت درمان با لیزر کمتر از سمت کنترل بود.
کلید واژه‌ها: لیزر کم توان؛ اینترلوکین-17؛ مایع شیار لثه‌ای؛ ارتودنسی. 

عنوان مقاله [English]

Effect of Low-Level Laser on IL-17 Level in GCF during Orthodontic Treatment

چکیده [English]

Introduction: Considering the role of inflammatory mediators in both orthodontic movements and the development of periodontal diseases and taking into account the role of Interleukin 17 as an important inflammatory mediator in orthodontic treatments and the impact of low-level laser therapy (LLLT) as a new and appropriate adjuvant therapy, the aim of the present study was to investigate the effect of LLLT on IL-17 levels in gingival cervicular fluid (GCF) during orthodontic treatment.
Materials and Methods: In this clinical trial study, 12 patients in need of orthodontic treatment were selected. In these patients, one side of the mouth was randomly selected for laser irradiation and the other side as a control. Diode LLL irradiation with a wavelength of 810 nm was performed throughout the gingival Cervicular on days 0, 2, 18, and 30 after orthodontic treatment. Sampling was performed on days 0, 2, 18, and 30 by placing paper points in distal, mesial, palatal, and buccal sulcus of the maxillary fifth molar on the both sides for 30 seconds. All samples were then transferred to the laboratory and IL-17 level was determined by ELISA reader. Data were analyzed using Paired t-test and One-way ANOVA.
Results: Mean IL-17 levels at day zero (p value = 0.62) and day 2 after orthodontic treatment (p value = 0.19) were not significantly different between the control and laser treated sides, however they were significantly lower in the laser-treated group than the control group on days 18 and 30 after orthodontic treatment (p value < 0.001). The mean IL-17 levels decreased in both groups during the treatment period, but this decrease was significant on day 18.
Conclusion: The results of the present study indicate the therapeutic role of diode LLL in reducing inflammation, and Il-17 levels in both sides of the mouth gradually decreased over time, and this decrease was more pronounced in the laser-treated side than in the control side.
Keywords: Low-level Laser; Interleukin-17; Gingival cervicular fluid; Orthodontics.

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مجله دانشکده دندان‌پزشکی اصفهان
دوره 19، شماره 2 - شماره پیاپی 2
تیر 1402
صفحه 159-160