Evaluation of Antimicrobial Effect of Resin Cements Containing Nano Particles on Cariogenic Bacteria of Streptococcus Mutans
Document Type : مقالههای پژوهشی
Authors
1 Professor, Department of Restorative Dentistry, school of Dentistry, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2 Associate Professor, Department of Restorative Dentistry, school of Dentistry, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
3 Associate Professor, Department of Microbiology, school of Medicine Shahid Sadoughi University of Medical Sciences, Yazd, Iran
4 Post Graduate Student, Department of Restorative Dentistry, school of Dentistry, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Abstract
Introduction: This study aimed to investigate the antibacterial properties of resin cement containing nano-titanium oxide particles and nano-chitosan particles on the cariogenic bacteria Streptococcus mutans.
Materials and methods: This experimental study was conducted at Yazd University in 2023 and included 60 samples divided into three groups. The control group samples were made of resin cement, and the second and third groups contained chitosan and titanium dioxide nanoparticles, respectively. For checking the antibacterial effects, 24 samples were placed on the Culture medium containing Streptococcus mutans, and the diameter of the non-growth halo of bacteria was measured. To investigate how time impacts the antibacterial activity of the samples, four samples from each of the three groups were placed in test tubes containing culture medium, and on days 1, 8, and 28, Streptococcus mutans was added to the test tube, and after culture on agar medium, the number of bacteria was reported. The data was analyzed using Kolmogorov-Smirnov and Independent samples T-test, Kruskal-Wallis Test non-parametric equivalents.
Result: The mean diameter of the non-growth halo in the control group was significantly lower than the other two groups (p value < 0.05). In investigating the effect of time on the antibacterial effect of the samples, on the first day, the average number of bacteria in resin cement containing titanium was lower than the other two groups, but this difference was not statistically significant. (p value < 0.05) The average number of bacteria on days 8 and 28 was the same in all three groups
Conclusion: Resin cement containing titanium and chitosan reduces the growth of bacteria in the vicinity of samples. Although the number of bacteria in the surroundings of these nanoparticles decreased on the first day, the long-term results indicated no difference between the groups.
Materials and methods: This experimental study was conducted at Yazd University in 2023 and included 60 samples divided into three groups. The control group samples were made of resin cement, and the second and third groups contained chitosan and titanium dioxide nanoparticles, respectively. For checking the antibacterial effects, 24 samples were placed on the Culture medium containing Streptococcus mutans, and the diameter of the non-growth halo of bacteria was measured. To investigate how time impacts the antibacterial activity of the samples, four samples from each of the three groups were placed in test tubes containing culture medium, and on days 1, 8, and 28, Streptococcus mutans was added to the test tube, and after culture on agar medium, the number of bacteria was reported. The data was analyzed using Kolmogorov-Smirnov and Independent samples T-test, Kruskal-Wallis Test non-parametric equivalents.
Result: The mean diameter of the non-growth halo in the control group was significantly lower than the other two groups (p value < 0.05). In investigating the effect of time on the antibacterial effect of the samples, on the first day, the average number of bacteria in resin cement containing titanium was lower than the other two groups, but this difference was not statistically significant. (p value < 0.05) The average number of bacteria on days 8 and 28 was the same in all three groups
Conclusion: Resin cement containing titanium and chitosan reduces the growth of bacteria in the vicinity of samples. Although the number of bacteria in the surroundings of these nanoparticles decreased on the first day, the long-term results indicated no difference between the groups.
Keywords
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