Journal of Isfahan Dental School

Antifungal Effect of Henna Methanolic Extract on Candida Albicans Strains Isolated from Patients with Oral Candidiasis

Document Type : مقاله‌های پژوهشی

Authors

1 Assistant Professor, Department of oral diseases, School of Dentistry, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

2 Assistant Professor, Department of Mirobiology, School of Dentistry, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

3 Postgraduate Dentistry, Department of prosthetic dentistry, School of Dentistry, Isfahan (Khorasgan) branch, Islamic Azad University, Isfahan, Iran

Abstract

Introduction: Several antifungal drugs have been proposed for the treatment of oral candidiasis, each of which has advantages and side effects. The use of medicinal plants as an antifungal agent can be helpful due to the reduction of side effects caused by drugs. This study aimed to determine the antifungal effect of methanolic extract of henna on Candida albicans strains.
Materials and Methods: In this experimental laboratory study, one standard strain and five clinical strains of Candida albicans were isolated from five patients with oral candidiasis. This study was conducted in 2023 at Isfahan Islamic Azad University. Cultured candida cells were prepared, and standard fungal suspensions with a concentration of 0.5 McFarland were prepared and cultured on the surface of the sabouraud dextrose agar culture medium. To the wells, 100 microliters of the prepared extract with concentrations of 0.6, 1.25, 2.5, 5, and 10 mg/ml were added, and the plates were incubated. To the wells of the microplate, 100 microliters of henna extract dilutions were added. Then, the diameter of the microorganism Non-Growth halo and turbidity caused by growth was measured and compared. Data were analyzed with Mann-Whitney, Kruskal-Wallis, and ANOVA statistical tests (α = 0.05).
Results: A Significant difference in the amount of turbidity caused by growth between different concentrations of extract, nystatin, and positive control in strains of Candida albicans was observed (p value < 0.05). There was a significant difference in growth inhibition percentage between different concentrations of extract, nystatin, and positive control in strains of Candida albicans (p value < 0.05).
Conclusion: The antifungal effect of henna extract in concentrations of 1.25, 2.5, 5, and 10 mg/ml was positive against Candida albicans isolated from patients.
Keywords: Lawsonia plant; Candida albicans; Antifungal agents

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Journal of Isfahan Dental School
Volume 20, Issue 4 - Serial Number 4
February 2025