Comparison of the effect of three collagen cross-linking agents on microtensile bond Strength of composite resin to dentin
Document Type : مقالههای پژوهشی
Authors
1 Specialist of Aesthetic and Restorative Dentistry, Ahvaz, Iran
2 Department of Aesthetic and Restorative Dentistry, Faculty of Dentistry, Isf.C, Islamic Azad University, Isfahan, Iran
3 Dentist, School of Dentistry, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran
10.22122/jids.2025.45269.1000
Abstract
Introduction: Achieving a strong and stable bond between resin composite and dentin remains a subject of debate in restorative dentistry. The aim of this study was to evaluate the effects of glutaraldehyde, hesperidin, and epigallocatechin gallate (EGCG) as collagen cross-linking agents on dentin bond strength by enhancing the stability of collagen fibers.
Materials and Methods: In this in vitro experimental study, 48 sound human molars were collected. After preparation to expose the dentin surface, the teeth were etched with 35% phosphoric acid. The specimens were randomly divided into four groups: 1. application of 2% hesperidin, 2. application of 5% glutaraldehyde, 3. application of 0.02% EGCG, and 4. control group (no treatment). After restoration with composite, the microtensile bond strength (μTBS) was evaluated. Data were analyzed using one-way ANOVA and Tukey's post hoc test.
Results: The μTBS in the EGCG-etched samples was higher than in samples etched with the other cross-linking agents; however, no significant difference was found between the groups treated with collagen cross-linkers (glutaraldehyde, hesperidin, and EGCG) and the untreated control group. A significant difference in bond strength was observed between the hesperidin and glutaraldehyde groups (P = 0.023), and between the hesperidin and EGCG groups (P = 0.005), while no significant differences were found between any of the treatment groups and the control group.
Conclusion: The application of collagen cross-linkers did not increase the microtensile bond strength of composite to dentin.
Materials and Methods: In this in vitro experimental study, 48 sound human molars were collected. After preparation to expose the dentin surface, the teeth were etched with 35% phosphoric acid. The specimens were randomly divided into four groups: 1. application of 2% hesperidin, 2. application of 5% glutaraldehyde, 3. application of 0.02% EGCG, and 4. control group (no treatment). After restoration with composite, the microtensile bond strength (μTBS) was evaluated. Data were analyzed using one-way ANOVA and Tukey's post hoc test.
Results: The μTBS in the EGCG-etched samples was higher than in samples etched with the other cross-linking agents; however, no significant difference was found between the groups treated with collagen cross-linkers (glutaraldehyde, hesperidin, and EGCG) and the untreated control group. A significant difference in bond strength was observed between the hesperidin and glutaraldehyde groups (P = 0.023), and between the hesperidin and EGCG groups (P = 0.005), while no significant differences were found between any of the treatment groups and the control group.
Conclusion: The application of collagen cross-linkers did not increase the microtensile bond strength of composite to dentin.
Keywords
- Al-Ammar A, Drummond JL, Bedran-Russo AK. The use of collagen cross-linking agents to enhance dentin bond strength. J Biomed Mater Res B Appl Biomater 2009; 91(1): 419-24.
- Islam S, Hiraishi N, Nassar M, Yiu C, Otsuki M, Tagami J. Effect of natural cross-linkers incorporation in a self-etching primer on dentine bond strength. J Dent 2012; 40(12): 1052-9.
- Zhang SC, Kern M. The role of host-derived dentinal matrix metalloproteinases in reducing dentin bonding of resin adhesives. Int J Oral Sci 2009; 1(4): 163-76
- Monticelli F, Toledano M, Silva AS, Osorio E, Osorio R. Sealing effectiveness of etch-and-rinse vs self-etching adhesives after water aging: influence of acid etching and NaOCl dentin pretreatment. J Adhes Dent 2008; 10(3): 183-8.
- Hashimoto M, Fujita S, Endo K, Ohno H. In vitro degradation of resin-dentin bonds with one-bottle self-etching adhesives. Eur J Oral Sci 2009; 117(5): 611-7.
- van Dijken JWV, Sunnegårdh-Grönberg K, Lindberg A. Clinical long-term retention of etch-and-rinse and self-etch adhesive systems in non-carious cervical lesions. A 13 years evaluation. Dent Mater 2007; 23(9): 1101-7.
- Tay FR, Pashley DH, Suh BI, Carvalho RM, Itthagarun A. Single-step adhesives are permeable membranes. J Dent 2002; 30(7-8): 371-82.
- Hashimoto M, Ohno H, Sano H, Kaga M, Oguchi H. In vitro degradation of resin-dentin bonds analyzed by microtensile bond test, scanning and transmission electron microscopy. Biomaterials 2003; 24(21): 3795-803.
- Malacarne J, Carvalho RM, de Goes MF, et al. Water sorption/solubility of dental adhesive resins. Dent Mater 2006; 22(10): 973-80.
- Pashley DH, Tay FR, Yiu C, Hashimoto M, Breschi L, Carvalho RM, et al. Collagen degradation by host-derived enzymes during aging. J Dent Res 2004; 83(3): 216-21.
- Hashimoto M, Ohno H, Kaga M, Endo K, Sano H, Oguchi H. In vivo degradation of resin-dentin bonds in humans over 1 to 3 years. J Dent Res 2000; 79(6): 1385-91.
- Marshall GW Jr, Marshall SJ, Kinney JH, Balooch M. The dentin substrate: structure and properties related to bonding. J Dent 1997; 25(6): 441-58.
- Yamauchi M, Shiiba M. Lysine hydroxylation and crosslinking of collagen. Methods Mol Biol 2002; 194: 277-90.
- Yang H, Guo J, Deng D, Chen Z, Huang C. Effect of adjunctive application of epigallocatechin-3-gallate and ethanol-wet bonding on adhesive-dentin bonds. J Dent 2016; 44: 44-9.
- Chen H, Sun G, Wang H, Yu S, Tian Z, Zhu S. Effect of collagen cross-linkers on dentin bond strength: A systematic review and network meta-analysis. Front Bioeng Biotechnol 2023; 10: 1100894.
- Bedran-Russo AKB, Pereira PNR, Duarte WR, Drummond JL, Yamauchi M. Application of crosslinkers to dentin collagen enhances the ultimate tensile strength. J Biomed Mater Res B Appl Biomater 2007; 80(1): 268-72.
- De Munck J, Mine A, Poitevin A, Van Ende A, Vivan Cardoso M, Van Landuyt KL, et al. Meta-analytical review of parameters involved in dentin bonding. J Dent Res 2012; 91(4): 351-7.
- Montagner AF, Sarkis-Onofre R, Pereira-Cenci T, Cenci MS. MMP Inhibitors on Dentin Stability: A Systematic Review and Meta-analysis. J Dent Res 2014; 93(8): 733-43
- Epasinghe DJ, Yiu CK, Burrow MF, Tsoi JK, Tay FR. Effect of flavonoids on the mechanical properties of demineralised dentine. J Dent 2014; 42(9): 1178-84.
- Srinivasulu S, Vidhya S, Sujatha M, Mahalaxmi S. Shear bond strength of composite to deep dentin after treatment with two different collagen cross-linking agents at varying time intervals. Oper Dent 2012; 37(5): 485-91
- Mapar M, Zakavi F, Farkhondeh Aghideh N, Rahmani Shahriari F, Siahposh A. The comparison of effect of chlorhexidine and proanthocyanidin and grape seed extract on composite-dentin micro tensile bond strength: an in vitro experimental study [in Persian]. Jundishapur Sci Med J 2016; 15(3): 283-92.
- Pashley DH, Tay FR, Yiu C, Hashimoto M, Breschi L, Carvalho RM, et al. Collagen degradation by host-derived enzymes during aging. J Dent Res 2004; 83(3): 216-21.
- Kim J, Gu L, Breschi L, Tjäderhane L, Choi KK, Pashley DH, et al. Implication of ethanol wet-bonding in hybrid layer J Dent Res 2010; 89(6): 575-80.
- Hardan L, Daood U, Bourgi R, Cuevas-Suárez CE, Devoto W, Zarow M, et al. Effect of collagen crosslinkers on dentin bond strength of adhesive systems: a systematic review and meta-analysis. Cells 2022; 11(15): 2417.
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