Comparison of the effect of three collagen cross-linking agents on microtensile bond Strength of composite resin to dentin

Document Type : مقاله‌های پژوهشی

Authors

1 Department of Restorative Dentistry, School of Dentistry, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran

2 Department of Operative Dentistry, Isf.c, Islamic Azad University, Isfahan, Iran

3 Dentis

10.22122/jids.2025.45269.1000

Abstract

Introduction: Achieving a strong and durable bond between resin composite and dentin remains a challenging and widely debated issue in restorative dentistry. The aim of this study was to evaluate the effects of glutaraldehyde, hesperidin, and epigallocatechin gallate (EGCG) as collagen cross-linking agents on the microtensile bond strength (μTBS) to dentin by enhancing the stability of collagen fibrils.
Materials and Methods: In this in vitro experimental study, 48 sound human molars were collected. After preparing the teeth to expose the dentin surface, they were etched with 35% phosphoric acid. The specimens were randomly divided into four groups: (1) 2% hesperidin, (2) 5% glutaraldehyde, (3) 0.02% EGCG, and (4) control group without any treatment. Following adhesive procedures and composite restoration, the microtensile bond strength (μTBS) was measured. Data were analyzed using one-way ANOVA and Tukey post hoc test.
Results: The μTBS in the EGCG-treated group was higher than in the other cross-linker-treated groups; however, the difference was not statistically significant compared to the untreated control group. A significant difference in bond strength was observed between the hesperidin and glutaraldehyde groups (P = 0.023), and between the hesperidin and EGCG groups (P = 0.005), while no significant differences were found between any of the treatment groups and the control group.
Conclusion: The application of collagen cross-linkers such as glutaraldehyde, hesperidin, and EGCG did not significantly enhance the microtensile bond strength of composite resin to dentin

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