فراوانی سلول‌های لانگرهانس در کارسینوم سلول سنگفرشی دهان با و بدون درگیری لنف نودهای گردنی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دندانپزشک، کمیته تحقیقات دانشجویی، دانشکده‌ی دندانپزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

2 دانشیار، مرکز تحقیقات دندانپزشکی، گروه پاتولوژی دهان، فک و صورت، دانشکده‌ی دندانپزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

3 دانشیار، مرکز تحقیقات مواد دندانی، گروه پاتولوژی دهان، فک و صورت، دانشکده‌ی دندانپزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

10.22122/jids.2025.45608.1018

چکیده

مقدمه: اغلب بیماران مبتلا به کارسینوم سلول سنگفرشی دهان (Oral Squamous Cell Carcinoma) OSCC در زمان تشخیص، بیماری پیشرفته‌ای دارند. مطالعه‌ی حاضر با هدف بررسی مقایسه‌ای فراوانی سلول‌های لانگرهانس در OSCC با و بدون درگیری لنف نودهای گردنی انجام شد.
مواد و روش‌ها: ددر مطالعه‌ی توصیفی- تحلیلی، از نوع مقطعی حاضر که در دانشکده‌ی دندانپزشکی در سال 1404 انجام شد، به بررسی 20 نمونه OSCC با درگیری لنف نودهای گردنی (گروه 1) و 20 نمونه OSCC بدون درگیری لنف نودهای گردنی (گروه 2) ثبت شده در آرشیو بخش آسیب‌شناسی دهان دانشکده‌ی دندانپزشکی اصفهان و بیمارستان آیت الله کاشانی پرداخت. جهت تعیین تعداد سلول‌های لانگرهانس، نمونه‌ها تحت رنگ آمیزی ایمنوهیستوشیمی با مارکر  CD1a قرار گرفت و تعداد سلول‌های لانگرهانس در تومور تعیین شد. اطلاعات حاصل با استفاده از آزمون های T، Chi-square، Mann-Whitney و Fisher مورد تحلیل آماری قرار گرفت. میزان 0/05P <  معنی‌دار تلقی شد.
یافته‌ها: میانگین تعداد سلول‌های لانگرهانس در گروه 1 به صورت معنی‌داری کمتر از گروه 2بود (0/001 > P). میانگین تعداد این سلول‌ها بر اساس سن، جنسیت، اندازه‌ی تومور، تهاجم عصبی، درجه بدخیمی (Grade) در دو گروه، اختلاف معنی‌داری نداشت (0/05 > P). میانگین تعداد سلول‌های لانگرهانس بر اساس تهاجم لنفووسکولار و مرحله‌ی بالینی (Stage) اختلاف معنی‌دار نشان داد (0/001 > P).
نتیجه‌گیری: کاهش معنی‌دار تعداد سلول‌های لانگرهانس با درگیری لنف نودهای گردنی در ارتباط است. لذا بررسی این سلول‌ها می‌تواند جهت تعیین پیش‌آگهی و تعیین روش‌های درمانی مناسب‌تر کمک کننده باشد.

تازه های تحقیق

لاله ملکی: PubMed, Google Scholar

سعیده خالصی: PubMed, Google Scholar

کلیدواژه‌ها

عنوان مقاله [English]

Frequency of Langerhans Cells in Oral Squamous Cell Carcinoma with and without Cervical Lymph Node Involvement

نویسندگان [English]

  • Ali Jamshidi 1
  • Laleh Maleki 2
  • Saeedeh Khalesi 3
1 Dentist, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran
2 Associate Professor, Dental Research Center, Department of Oral and Maxillofacial Pathology, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran
3 Associate Professor, Dental Materials Research Center, Department of Oral and Maxillofacial Pathology, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده [English]

Introduction: Most patients with oral squamous cell carcinoma (OSCC) are diagnosed at an advanced stage. The present study aimed to compare the frequency of Langerhans cells in OSCC with and without cervical lymph node involvement.
Materials and Methods: In this descriptive-analytical, cross-sectional study conducted at the School of Dentistry in 2024-2025, 20 OSCC samples with cervical lymph node involvement (group 1) and 20 OSCC samples without cervical lymph node involvement (group 2) were selected from the archives of the Oral Pathology Department of Isfahan Dental School and Ayatollah Kashani Hospital. To determine the number of Langerhans cells, the samples underwent immunohistochemical staining for the CD1a marker, and the number of Langerhans cells within the tumor was counted. The obtained data were statistically analyzed using independent t-tests, chi-square, Mann-Whitney and Fisher's exact tests. P < 0.05 was considered significant.
Results: The mean number of Langerhans cells in group 1 was significantly lower than that in group 2 (P < 0.001). The mean number of these cells did not differ significantly in the two groups based on age, gender, tumor size, neural invasion, and histopathological grade (P < 0.05). The mean number of Langerhans cells showed a significant difference based on lymphovascular invasion and clinical stage (P < 0.001).
Conclusion: A significant decrease in the number of Langerhans cells is associated with cervical lymph node involvement. Therefore, examining these cells can be helpful in determining prognosis and determining more appropriate treatment methods.

کلیدواژه‌ها [English]

  • Langerhans
  • Squamous cell carcinoma
  • Mouth
  • Immunohistochemistry

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مجله دانشکده دندان‌پزشکی اصفهان
دوره 21، شماره 3
دوره 21، شماره 3، مهر 1404 (در حال تکمیل)
مهر 1404

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