Comparative evaluation of CD105 protein expression in dysplastic and cancerous oral lesions by immunohistochemical staining
Document Type : مقالههای پژوهشی
Authors
1
Assistant Professor, Dental Materials Research Center, Department of Oral and Maxillofacial Pathology, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran
2
Assistant Professor, Dental Research Center, Department of Oral and Maxillofacial Pathology, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran
3
Dental Student, Dental Students Research Center, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
10.48305/v12i3.1003
Abstract
Introduction: Given the importance of angiogenesis and formation of new vessels and their relationship with the progression of malignant lesions and also CD105 sensitivity in determining newly formed vessels, the present study was undertaken to evaluate CD105 protein expression in dysplastic and malignant oral lesions.
Materials & Methods: In this cross-sectional descriptive‒analytical study 20 paraffin blocks from dysplastic oral lesions and 20 paraffin blocks from squamous cell carcinoma lesions based on histopathological criteria, from patients referring to the Department of Oral and Maxillofacial Pathology of the School of Dentistry in the last 10 years were selected using simple sampling technique. The samples were stained immunohistochemically for CD105 marker and evaluated by two pathologists simultaneously under a light microscope at ×400 magnification. The mean of stained areas was considered as the mean vessel density with CD105 expression in each sample. Data were analyzed with SPSS 20, using Mann-Whitney test (α=0.05).
Results: The means of CD105 marker expression in dysplastic and squamous cell carcinoma lesions were 7.58±6.19 and 19.35±8.63, respectively. Mann-Whitney test showed significant differences between the two groups in CD105 expression (p value < 0.001).
Conclusion: Given the importance of CD105 marker expression in new vessels and its significant increase in malignant lesions, it can be concluded that the progression of dysplastic lesions toward malignancy is accompanied by neoangiogenesis and this marker can be useful for evaluation of the course of dysplastic lesions or as a therapeutic aim for malignant lesions.
Key words: Dysplastic, Squamous cell carcinoma. Staining.
Materials & Methods: In this cross-sectional descriptive‒analytical study 20 paraffin blocks from dysplastic oral lesions and 20 paraffin blocks from squamous cell carcinoma lesions based on histopathological criteria, from patients referring to the Department of Oral and Maxillofacial Pathology of the School of Dentistry in the last 10 years were selected using simple sampling technique. The samples were stained immunohistochemically for CD105 marker and evaluated by two pathologists simultaneously under a light microscope at ×400 magnification. The mean of stained areas was considered as the mean vessel density with CD105 expression in each sample. Data were analyzed with SPSS 20, using Mann-Whitney test (α=0.05).
Results: The means of CD105 marker expression in dysplastic and squamous cell carcinoma lesions were 7.58±6.19 and 19.35±8.63, respectively. Mann-Whitney test showed significant differences between the two groups in CD105 expression (p value < 0.001).
Conclusion: Given the importance of CD105 marker expression in new vessels and its significant increase in malignant lesions, it can be concluded that the progression of dysplastic lesions toward malignancy is accompanied by neoangiogenesis and this marker can be useful for evaluation of the course of dysplastic lesions or as a therapeutic aim for malignant lesions.
Key words: Dysplastic, Squamous cell carcinoma. Staining.
