نوع مقاله : Original Articles
نویسندگان
1 استادیار، مرکز تحقیقات مواد دندانی، گروه اندودونتیکس، دانشکده دندانپزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
2 دانشجوی دندانپزشکی، کمیته پژوهشهای دانشجویی، دانشکده دندانپزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
3 استاد، مرکز تحقیقات مواد دندانی، گروه اندودونتیکس، دانشکده دندانپزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
4 کارشناس میکروبیولوژی، مرکز تحقیقات پروفسور ترابینژاد، دانشکده دندانپزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
چکیده
عنوان مقاله [English]
Introduction: One of the important characteristics of endodontic sealers is their antifungal activity. The purpose of this study was to compare the antifungal activity of following sealers: MTA Fillapex, tg-sealer, Multi-Cal, AH-Plus, RoekoSeal against Candida albicans, by two different direct and indirect methods. Materials and methods: In this experimental study, the agar diffusion test (ADT) (the indirect method) was used to compare the antifungal activity of freshly mixed sealers against a standard strain of C. albicans. Each sealer was placed in a well prepared in the middle of agar plates, in contact with C. albicans. After incubation, the diameter of the zone of inhibition of fungal growth was measured by caliper. In direct contact test (DCT), to evaluate the antifungal activity of sealers after setting, suspensions containing the powdered sealer and C. albicans were cultivated on agar plates after 6, 15 and 60 minutes and incubated. Colony-forming units (CFU/mL) were calculated after incubation. Data were analyzed with SPSS 20 using one-way ANOVA, repeated-measures ANOVA and t-test (α = 0.05). Results: ADT method for freshly mixed sealers showed that the antibacterial activity of Multi-Cal against C. albicans was significantly higher than the other sealers (p value < 0.001). However, in DCT method for set sealers, MTA Fillapex exhibited the maximum antifungal effect against C. albicans (p value < 0.05). The silicone sealer RoekoSeal had the least antifungal activity in both techniques (p value < 0.05). Conclusion: The sealers Multi-Cal and MTA Fillapex exhibited the highest with ADT and DCT techniques, respectively. RoekoSeal exhibited the least antifungal activity with both techniques. Key words: Candida albicans, Root canal therapy, Sealer