Cytotoxicity Effects of Polyhydroxybutyrate/Chitosan/Bioglass Nanocomposite Scaffolds on Human Osteoblast-like Cells (SAOS-2 Cells)

Document Type : مقاله‌های پژوهشی

Authors

1 Professor, Dental Research Center, Department of Anatomical Sciences, Dental Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran

2 Professor, Dental Materials Research Center, Department of Operative Dentistry, Dental Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran

3 Professor, Department of Biomaterials and Tissue Engineering, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

4 Bachelor of Sciences Microbiology, Dental Research Center, Dental Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran

5 PhD Student, Dental Materials Research Center, Dental Research Institute/, Isfahan University of Medical Sciences, Isfahan, Iran

10.48305/v0i0.1677

Abstract

Introduction: In tissue engineering, a porous material is applied as the extracellular matrix or scaffold for cell growth. Then growth factors are added to this scaffold whose cytotoxicity must be assessed. In this study, the cytotoxicity effects of polyhydroxybutyrate/chitosan/bioglass nanocomposite scaffolds on human osteoblast-like cells (SAOS-2 cells) are explored.
Materials and Methods: This study was experimental and was done in Isfahan Dental School. The polyhydroxybutyrate/chitosan/bioglass nanocomposite scaffold was fabricated using the electrospinning method. Scanning Electron Microscopy (SEM) analysis was used to evaluate the morphological characteristics of the nanofibers and their diameter distribution. Additionally, bioglass nanoparticles in the fibers were identified via SEM. The MTT assay was performed to assess cell viability after 3, 5, and 7 days. The collected data were analyzed using SPSS 20.0 through statistical tests including Kruskal-Wallis test (p value ≤ 0.05).
Results: According to SEM images, the scaffold fibers were fully porous and no beads were observed. Furthermore, the polyhydroxybutyrate/chitosan/bioglass scaffold showed greater cell viability and proliferation compared to the groups lacking bioglass.
Conclusion: The polyhydroxybutyrate/chitosan/bioglass scaffold has no cytotoxic effect on osteoblast– like cells.
Keywords: HHydroxybutyrate; Bioglass; Chitosan; Osteoblast; Cytotoxicity.

References

1. Cai X, Lin Y, Ou G, Luo E, Man Y, Yuan Q, et al. Ectopic osteogenesis and chondrogenesis of bone marrow stromal stem cells in alginate system. Cell Biol Int 2007; 31(8): 776-7.
2. Kurashina K, Kurita H, Wu Q, Ohtsuka A, Kobayashi H. Ectopic osteogenesis with biphasic ceramics of hydroxyapatite and tricalcium Phosphate in rabbits. Biomaterial 2002; 23(2): 407-12.
3. Misra SK, Valappil SP, Roy I, Boccaccini AR. Polyhydroxyalkanoate (PHA)/inorganic phase composites for tissue engineering applications. Biomacromolecules 2006; 7(8): 2249-58.
4. Jacquel N, Lo CW, Wei YH, Wu HS, Wang SS. Isolation and purification of bacterial poly(3-hydroxyalkanoates. Biochem Eng J 2008; 39(1): 15-27.
5. Doyle C, Tanner ET, Bonfield W. In vitro and in vivo evaluation of polyhydroxybutyrate and of polyhydroxybutyrate reinforced with hydroxyapatite. Biomaterials 1991; 12(9): 841-7.
6. Luklinska ZB, Schluckwerder H. In vivo response to HA-polyhydroxybutyrate/polyhydroxyvalerate composite. J Microsc 2003; 211(Pt 2) :121-9.
7. Ravi Kumar MNV. A review of chitin and chitosan applications. React Funct Polym 2000; 46(1): 1-27.
8. Goy RC, de Britto D, Assis OBG. A review of the antimicrobial activity of chitosan. Polimeros 2009; 19(3): 241-7.
9. Sombatmankhong K, Suwantong O, Waleetorncheepsawat S, Supaphol P. Electrospun fiber mats of poly(3-hydroxybutyrate), poly(3-hydroxybutyrate-co-3-hydroxyvalerate), and their blends. J Polym Sci B Polym Phys 2006; 44(19): 2923-33.
10. Daranarong D, Chan RTH, Wanandy NS, Molloy R, Punyodom W, Foster LJR. Electrospun polyhydroxybutyrate and poly(L-lactide-co-ε-caprolactone) composites as nanofibrous scaffolds. Biomed Res Int 2014; 2014: 741408.
11. Foroughi MR, Karbasi S, Khoroushi M, Khademi AA. Polyhydroxybutyrate/chitosan/bioglass nanocomposite as a novel electrospun scaffold: fabrication and characterization. J Porous Mater 2017; 24(6): 1447-60.
12. Luklinska ZB, Schluckwerder H. In vivo response to HA-polyhydroxybutyrate/ polyhydroxyvalerate composite. J Microsc 2003; 211(Pt 2): 121-9.
13. Deniz AE. Nanofibrous nanocomposites via electrospining. [Thesis]. Ankara, Turkey: Bilkent University; 2011.
14. Kim HW, Lee HH, Knowles JC. Electrospinning biomedical nanocomposite fibers of hydroxyapatite/poly (lactic acid) for bone regeneration. J Biomed Mater Res A 2006; 79(3): 643-9.
15. Zhang Y, Ouyang H, Lim CT, Ramakrishna S, Huang ZM. Electrospinning of gelatin fibers and gelatin/PCL composite fibrous scaffolds. J Biomed Mater Res B Appl Biomater 2005; 72(1): 156-65.
16. Yang X, Yang F, Walboomers XF, Bian Z, Fan M, Jansen JA. The performance of dental pulp stem cells on nanofibrous PCL/gelatin/nHA scaffolds. J Biomed Mater Res A 2010; 93(1): 247-57.
Journal of Isfahan Dental School
Volume 18, Issue 4 - Serial Number 4
December 2021
Pages 423-430

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