Journal of Isfahan Dental School

Effect of Low-Level Laser on IL-17 Level in GCF during Orthodontic Treatment

Document Type : مقاله‌های پژوهشی

Authors

1 Assistant Professor, Department of Orthodontics, School of Dentistry ,Isfahan (Khorasgan) Branch ,Islamic Azad University, Isfahan, Iran

2 Assistant Professor, Department of Orthodontics, School of Dentistry ,Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

3 Postgraduate Student, Department of Periodontics, School of Dentistry, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

Abstract

Introduction: Considering the role of inflammatory mediators in both orthodontic movements and the development of periodontal diseases and taking into account the role of Interleukin 17 as an important inflammatory mediator in orthodontic treatments and the impact of low-level laser therapy (LLLT) as a new and appropriate adjuvant therapy, the aim of the present study was to investigate the effect of LLLT on IL-17 levels in gingival cervicular fluid (GCF) during orthodontic treatment.
Materials and Methods: In this clinical trial study, 12 patients in need of orthodontic treatment were selected. In these patients, one side of the mouth was randomly selected for laser irradiation and the other side as a control. Diode LLL irradiation with a wavelength of 810 nm was performed throughout the gingival Cervicular on days 0, 2, 18, and 30 after orthodontic treatment. Sampling was performed on days 0, 2, 18, and 30 by placing paper points in distal, mesial, palatal, and buccal sulcus of the maxillary fifth molar on the both sides for 30 seconds. All samples were then transferred to the laboratory and IL-17 level was determined by ELISA reader. Data were analyzed using Paired t-test and One-way ANOVA.
Results: Mean IL-17 levels at day zero (p value = 0.62) and day 2 after orthodontic treatment (p value = 0.19) were not significantly different between the control and laser treated sides, however they were significantly lower in the laser-treated group than the control group on days 18 and 30 after orthodontic treatment (p value < 0.001). The mean IL-17 levels decreased in both groups during the treatment period, but this decrease was significant on day 18.
Conclusion: The results of the present study indicate the therapeutic role of diode LLL in reducing inflammation, and Il-17 levels in both sides of the mouth gradually decreased over time, and this decrease was more pronounced in the laser-treated side than in the control side.
Keywords: Low-level Laser; Interleukin-17; Gingival cervicular fluid; Orthodontics.

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Journal of Isfahan Dental School
Volume 19, Issue 2 - Serial Number 2
July 2023
Pages 159-160