Evaluation of the Effect of Contamination with Blood and Homeostatic Agents on the Dentin Bond Strength of Primary Teeth

Document Type : Original Articles

Authors

1 Postgraduate Student, Department of Pediatric Dentistry, School of Dentistry, Isf.C, Islamic Azad University, Isfahan, Iran

2 Assistant Professor, Department of Pediatric Dentistry, School of Dentistry, Isf.C, Islamic Azad University, Isfahan, Iran

3 Associate Professor. Department of Operative Dentistry, School of Dentistry, Isf.C, Islamic Azad University, Isfahan, Iran

4 Postgraduate Student, Department of Operative Dentistry, School of Dentistry, Medical University of Isfahan, Isfahan, Iran

10.22122/jids.v21.i3.0208

Abstract

Introduction: Contamination is a frequent challenge during composite restorations in pediatric dentistry, often compromising the bond strength of restorative materials. This study aimed to evaluate the effect of blood and hemostatic agents contamination on the bond strength of primary teeth.
Materials and Methods: In this in vitro experimental study, 80 extracted primary teeth collected in 2020 from the Islamic Azad University, Isfahan (Khorasgan) Branch, were used. The samples were divided into 4 groups: contaminated with blood, contaminated with 25% Aluminum chloride, contaminated with 15.5% Ferric sulfate, and no contamination (control group). Each group was subdivided into two subgroups based on the bonding system used (self-etch [SE bond] and universal [G premium]), and the composite was placed and cured. Bond strength was measured using a universal testing machine, and the final breaking force was recorded. Data were analyzed using the Kruskal-Wallis parametric test (α = 0.05).
Results: Two-way ANOVA results showed that both contamination type and bonding type had a significant effect on dentin bond strength, while the interaction between these two factors was not significant. The SE Bond system generally performed better than G-Premium. Among contamination types, the control group had the highest bond strength, and the group contaminated with 25% Aluminum Chloride showed the lowest..
Conclusion: Contamination with blood and hemostatic agents (25% aluminum chloride and 15.5% ferric sulfate) significantly reduces dentin bond strength in both SE Bond and G-Premium.

Highlights

Fatemeh Rezaei:PubMed, Google Scholar

Shahrzad JavadiNejad: PubMed, Google Scholar

Parvin MirzaKouchaki:  PubMed, Google Scholar

Negar Rezapour: PubMed, Google Scholar

Keywords

References

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Journal of Isfahan Dental School
Volume 21, Issue 3
Volume 21, Issue 3, October 2025
October 2025
Pages 208-217

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